› 1xbr

X-ray diffraction
2.5Å resolution

T DOMAIN FROM XENOPUS LAEVIS BOUND TO DNA

Released:
DOI: 10.2210/pdb1xbr/pdb
PDB entry 1xbr coloured by chain, front view.
PDB entry 1xbr coloured by chain, side view.
PDB entry 1xbr coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Crystallographic structure of the T domain-DNA complex of the Brachyury transcription factor.
Müller CW, Herrmann BG
Nature 389 884-8 (1997)
PMID: 9349824

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114802 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
T-box transcription factor T Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.07 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24781 (Residues: 39-222; Coverage: 43%)
Gene names: bra, t, tbxt
Sequence domains: T-box
Structure domains: Transcription factor, T-box
DNA (5'-D(*AP*AP*TP*TP*TP*CP*AP*CP*AP*CP*CP*TP*AP*GP*GP*TP*G P*TP*GP*AP*AP*AP* TP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 37.9Å b: 113.9Å c: 149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

36 review citations

T-box genes in vertebrate development.
Naiche et al. (2005)
35 more

14 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
13 more