› 1xcm

X-ray diffraction
1.84Å resolution

Crystal structure of the GppNHp-bound H-Ras G60A mutant

Released:
DOI: 10.2210/pdb1xcm/pdb
PDB entry 1xcm coloured by chain, front view.
PDB entry 1xcm coloured by chain, side view.
PDB entry 1xcm coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ford B, Nassar N

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-167; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GNP 1 x GNP
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 34.292Å b: 81.419Å c: 121.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.244
Expression system: Escherichia coli BL21(DE3)

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