› 1xdi

X-ray diffraction
2.81Å resolution

Crystal structure of LpdA (Rv3303c) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1xdi/pdb
PDB entry 1xdi coloured by chain, front view.
PDB entry 1xdi coloured by chain, side view.
PDB entry 1xdi coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Characterization of a new member of the flavoprotein disulfide reductase family of enzymes from Mycobacterium tuberculosis.
Argyrou A, Vetting MW, Blanchard JS
J Biol Chem 279 52694-702 (2004)
PMID: 15456792

Function and Biology Details

Reaction catalysed: 
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H dehydrogenase (quinone) Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 52.37 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHH7 (Residues: 1-493; Coverage: 100%)
Gene names: Rv3303c, lpdA, lpdA-2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R32
Unit cell:
a: 244.2Å b: 244.2Å c: 104.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.276
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Lipoic acid metabolism in microbial pathogens.
Spalding et al. (2010)
7 other articles

6 mentions without citation

A unified statistical model to support local sequence order independent similarity searching for ligand-binding sites and its application to genome-based drug discovery.
Xie et al. (2009)
5 more