› 1xdp

X-ray diffraction
2.5Å resolution

Crystal Structure of the E.coli Polyphosphate Kinase in complex with AMPPNP

Released:
DOI: 10.2210/pdb1xdp/pdb
PDB entry 1xdp coloured by chain, front view.
PDB entry 1xdp coloured by chain, side view.
PDB entry 1xdp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.
Zhu Y, Huang W, Lee SS, Xu W
EMBO Rep 6 681-7 (2005)
PMID: 15947782

Function and Biology Details

Reaction catalysed: 
ATP + (phosphate)(n) = ADP + (phosphate)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-141572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 687 amino acids
Theoretical weight: 80.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7B1 (Residues: 2-688; Coverage: 100%)
Gene names: JW2486, b2501, ppk
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42212
Unit cell:
a: 152Å b: 152Å c: 150Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.337 0.248 0.274
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Inorganic polyphosphate: essential for growth and survival.
Rao et al. (2009)
12 more

11 mentions without citation

Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.
Zhu et al. (2005)
10 more