› 1xeq

X-ray diffraction
2.1Å resolution

Crystal tructure of RNA binding domain of influenza B virus non-structural protein

Released:
DOI: 10.2210/pdb1xeq/pdb
PDB entry 1xeq coloured by chain, front view.
PDB entry 1xeq coloured by chain, side view.
PDB entry 1xeq coloured by chain, top view.
Source organism: Influenza B virus (B/Lee/1940)
Primary publication:
Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses.
Yin C, Khan JA, Swapna GV, Ertekin A, Krug RM, Tong L, Montelione GT
J Biol Chem 282 20584-92 (2007)
PMID: 17475623

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-137084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.88 KDa
Source organism: Influenza B virus (B/Lee/1940)
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03502 (Residues: 1-103; Coverage: 37%)
Gene name: NS
Sequence domains: Influenza B non-structural protein (NS1)
Structure domains: S15/NS1, RNA-binding

Ligands and Environments

1 bound ligand:
Ligand BR 19 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P6422
Unit cell:
a: 102.032Å b: 102.032Å c: 108.843Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.256
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

The multifunctional NS1 protein of influenza A viruses.
Hale et al. (2008)
16 more

3 mentions without citation

Protein intrinsic disorder toolbox for comparative analysis of viral proteins.
Goh et al. (2008)
2 more