› 1xf1

X-ray diffraction
1.9Å resolution

Structure of C5a peptidase- a key virulence factor from Streptococcus

Released:
DOI: 10.2210/pdb1xf1/pdb
PDB entry 1xf1 coloured by chain, front view.
PDB entry 1xf1 coloured by chain, side view.
PDB entry 1xf1 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structure of the streptococcal cell wall C5a peptidase.
Brown CK, Gu ZY, Matsuka YV, Purushothaman SS, Winter LA, Cleary PP, Olmsted SB, Ohlendorf DH, Earhart CA
Proc Natl Acad Sci U S A 102 18391-6 (2005)
PMID: 16344483

Function and Biology Details

Reaction catalysed: 
The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C5a peptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 926 amino acids
Theoretical weight: 103 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P15926 (Residues: 106-1031; Coverage: 82%)
Gene name: scpA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand CA 2 x CA
Ligand CIT 6 x CIT
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 114.696Å b: 75.113Å c: 132.391Å
α: 90° β: 104.95° γ: 90°
R-values:
R R work R free
0.229 0.229 0.269
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Streptococcus adherence and colonization.
Nobbs et al. (2009)
9 more

4 mentions without citation

Structure of the streptococcal cell wall C5a peptidase.
Brown et al. (2005)
3 more