› 1xfj

X-ray diffraction
1.75Å resolution

Crystal structure of protein CC_0490 from Caulobacter crescentus, Pfam DUF152

Released:
DOI: 10.2210/pdb1xfj/pdb
PDB entry 1xfj coloured by chain, front view.
PDB entry 1xfj coloured by chain, side view.
PDB entry 1xfj coloured by chain, top view.
Source organism: Caulobacter vibrioides
Entry authors: Krishnamurthy NR, Kumaran D, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reactions catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.15 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AAV3 (Residues: 1-261; Coverage: 100%)
Gene name: CC_0490
Sequence domains: Multi-copper polyphenol oxidoreductase laccase
Structure domains: CNF1/YfiH-like putative cysteine hydrolases

Ligands and Environments

3 bound ligands:
Ligand ACT 1 x ACT
Ligand BME 1 x BME
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 39.807Å b: 66.828Å c: 43.17Å
α: 90° β: 110.6° γ: 90°
R-values:
R R work R free
0.18 0.18 0.22
Expression system: Escherichia coli

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