› 1xgk

X-ray diffraction
1.4Å resolution

CRYSTAL STRUCTURE OF N12G AND A18G MUTANT NMRA

Released:
DOI: 10.2210/pdb1xgk/pdb
PDB entry 1xgk coloured by chain, front view.
PDB entry 1xgk coloured by chain, side view.
PDB entry 1xgk coloured by chain, top view.
Source organism: Aspergillus nidulans
Primary publication:
Modulation of the ligand binding properties of the transcription repressor NmrA by GATA-containing DNA and site-directed mutagenesis.
Lamb HK, Ren J, Park A, Johnson C, Leslie K, Cocklin S, Thompson P, Mee C, Cooper A, Stammers DK, Hawkins AR
Protein Sci 13 3127-38 (2004)
PMID: 15537757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen metabolite repression protein nmrA Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 38.76 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5AU62 (Residues: 1-352; Coverage: 100%)
Gene names: AN8168, nmrA
Sequence domains: NmrA-like family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand K 4 x K
Ligand CL 5 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 76.61Å b: 76.61Å c: 103.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.193 0.238
Expression system: Escherichia coli

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Citations

4 review citations

Nitrogen regulation of fungal secondary metabolism in fungi.
Tudzynski B. (2014)
3 more