› 1xk5

X-ray diffraction
2.4Å resolution

Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide

Released:
DOI: 10.2210/pdb1xk5/pdb
PDB entry 1xk5 coloured by chain, front view.
PDB entry 1xk5 coloured by chain, side view.
PDB entry 1xk5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1.
Strasser A, Dickmanns A, Lührmann R, Ficner R
EMBO J 24 2235-43 (2005)
PMID: 15920472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Snurportin-1 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95149 (Residues: 97-300; Coverage: 57%)
Gene names: RNUT1, SNUPN, SPN1
Structure domains: DNA ligase/mRNA capping enzyme

Ligands and Environments

1 bound ligand:
Ligand TPG 1 x TPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 57.47Å b: 57.47Å c: 130.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.276
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structural biology of nucleocytoplasmic transport.
Cook et al. (2007)
6 more

10 mentions without citation

Applications of Phosphate Modification and Labeling to Study (m)RNA Caps.
Warminski et al. (2017)
9 more