› 1xm8

X-ray diffraction
1.74Å resolution

X-RAY STRUCTURE OF GLYOXALASE II FROM ARABIDOPSIS THALIANA GENE AT2G31350

Released:
DOI: 10.2210/pdb1xm8/pdb
PDB entry 1xm8 coloured by chain, front view.
PDB entry 1xm8 coloured by chain, side view.
PDB entry 1xm8 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural studies on a mitochondrial glyoxalase II.
Marasinghe GP, Sander IM, Bennett B, Periyannan G, Yang KW, Makaroff CA, Crowder MW
J Biol Chem 280 40668-75 (2005)
PMID: 16227621

Function and Biology Details

Reaction catalysed: 
S-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2-hydroxy carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyacylglutathione hydrolase 2, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 28.22 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SID3 (Residues: 72-324; Coverage: 78%)
Gene names: At2g31350, T28P16.16
Sequence domains:
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand FE 2 x FE
Ligand ACY 2 x ACY
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21
Unit cell:
a: 68.494Å b: 58.782Å c: 69.049Å
α: 90° β: 109.22° γ: 90°
R-values:
R R work R free
0.142 0.14 0.189
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Glutathione in plants: an integrated overview.
Noctor et al. (2012)
12 more

11 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
10 more