1xmz

X-ray diffraction
1.38Å resolution

Crystal structure of the dark state of kindling fluorescent protein kfp from anemonia sulcata

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-190440 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GFP-like non-fluorescent chromoprotein FP595 chain 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 amino acids
Theoretical weight: 8.07 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 2-62; Coverage: 26%)
GFP-like non-fluorescent chromoprotein FP595 chain 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 168 amino acids
Theoretical weight: 19.13 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 66-232; Coverage: 72%)
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 76.52Å b: 125.4Å c: 92.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.191
Expression system: Escherichia coli