1xo0

X-ray diffraction
2Å resolution

High resolution structure of the holliday junction intermediate in cre-loxp site-specific recombination

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-114821 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombinase cre Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.55 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-343; Coverage: 95%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
loxP Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.77 KDa
Source organism: Punavirus P1
Expression system: Not provided
loxP Chain: D
Molecule details ›
Chain: D
Length: 35 nucleotides
Theoretical weight: 10.74 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 107.4Å b: 123Å c: 180.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided