› 1xpp

X-ray diffraction
1.6Å resolution

Crystal Structure of TA1416,DNA-directed RNA polymerase subunit L, from Thermoplasma acidophilum

Released:
DOI: 10.2210/pdb1xpp/pdb
PDB entry 1xpp coloured by chain, front view.
PDB entry 1xpp coloured by chain, side view.
PDB entry 1xpp coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Entry authors: Kim Y, Joachimiak A, Evdokimova E, Savchenko A, Edwards A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit Rpo11 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 13.49 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HIC5 (Residues: 1-111; Coverage: 100%)
Gene names: Ta1416, rpo11, rpoL
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Structure domains: RNA polymerase, RBP11-like subunit

Ligands and Environments

3 bound ligands:
Ligand SCN 3 x SCN
Ligand ACY 1 x ACY
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 19-BM
Spacegroup: P2
Unit cell:
a: 59.7Å b: 69.424Å c: 66.293Å
α: 90° β: 116.77° γ: 90°
R-values:
R R work R free
0.224 0.219 0.264
Expression system: Escherichia coli BL21(DE3)

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