1xpx

X-ray diffraction
2.8Å resolution

Structural basis of prospero-DNA interaction; implications for transcription regulation in developing cells

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-114822 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein prospero Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 19.54 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29617 (Residues: 1541-1703; Coverage: 10%)
Gene names: CG17228, pros
Sequence domains: Homeo-prospero domain
Structure domains: Homeo-prospero domain
5'-D(*AP*GP*CP*AP*TP*GP*CP*CP*TP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
5'-D(*CP*AP*GP*GP*CP*AP*TP*GP*CP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P43212
Unit cell:
a: 44.708Å b: 44.708Å c: 226.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided