› 1xsd

X-ray diffraction
2.7Å resolution

Crystal structure of the BlaI repressor in complex with DNA

Released:
DOI: 10.2210/pdb1xsd/pdb
PDB entry 1xsd coloured by chain, front view.
PDB entry 1xsd coloured by chain, side view.
PDB entry 1xsd coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons.
Safo MK, Zhao Q, Ko TP, Musayev FN, Robinson H, Scarsdale N, Wang AH, Archer GL
J Bacteriol 187 1833-44 (2005)
PMID: 15716455

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114825 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Penicillinase repressor Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.99 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A042 (Residues: 1-126; Coverage: 100%)
Gene names: blaI, penI
Sequence domains: Penicillinase repressor
Structure domains:
5'-D(P*TP*AP*CP*TP*AP*CP*AP*TP*AP*TP*GP*TP*AP*GP*TP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I4122
Unit cell:
a: 72.141Å b: 72.141Å c: 243.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.23 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
8 more

10 mentions without citation

Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons.
Safo et al. (2005)
9 more