› 1xto

X-ray diffraction
2.8Å resolution

Crystal Structure of the Coenzyme PQQ Synthesis Protein (PqqB) from Pseudomonas putida, Northeast Structural Genomics Target PpR6

Released:
DOI: 10.2210/pdb1xto/pdb
PDB entry 1xto coloured by chain, front view.
PDB entry 1xto coloured by chain, side view.
PDB entry 1xto coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry authors: Forouhar F, Chen Y, Kuzin A, Vorobiev SM, Xiao R, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme PQQ synthesis protein B Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.95 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88QV5 (Residues: 1-303; Coverage: 100%)
Gene names: PP_0379, pqqB
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 86.051Å b: 86.051Å c: 110.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.282
Expression system: Escherichia coli

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