› 1xw8

X-ray diffraction
2Å resolution

X-ray structure of putative lactam utilization protein YBGL. Northeast Structural Genomics Consortium target ET90.

Released:
DOI: 10.2210/pdb1xw8/pdb
PDB entry 1xw8 coloured by chain, front view.
PDB entry 1xw8 coloured by chain, side view.
PDB entry 1xw8 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Kuzin AP, Chen Y, Vorobiev SM, Acton TB, Ma L-C, Xiao R, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + 5-oxo-L-proline + 2 H(2)O = ADP + phosphate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-oxoprolinase subunit A Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 27.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P75746 (Residues: 1-244; Coverage: 100%)
Gene names: JW0703, b0713, pxpA, ybgL
Sequence domains: LamB/YcsF family
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 72.669Å b: 37.208Å c: 79.801Å
α: 90° β: 93.97° γ: 90°
R-values:
R R work R free
0.241 0.241 0.265
Expression system: Escherichia coli

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