› 1xwg

X-ray diffraction
1.85Å resolution

Human GST A1-1 T68E mutant

Released:
DOI: 10.2210/pdb1xwg/pdb
PDB entry 1xwg coloured by chain, front view.
PDB entry 1xwg coloured by chain, side view.
PDB entry 1xwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New crystal structures of human glutathione transferase A1-1 shed light on glutathione binding and the conformation of the C-terminal helix.
Grahn E, Novotny M, Jakobsson E, Gustafsson A, Grehn L, Olin B, Madsen D, Wahlberg M, Mannervik B, Kleywegt GJ
Acta Crystallogr D Biol Crystallogr 62 197-207 (2006)
PMID: 16421451

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08263 (Residues: 2-222; Coverage: 100%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 99.077Å b: 90.57Å c: 51.489Å
α: 90° β: 93.06° γ: 90°
R-values:
R R work R free
0.169 0.164 0.218
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
4 more