› 1xwl

X-ray diffraction
1.7Å resolution

BACILLUS STEAROTHERMOPHILUS (NEWLY IDENTIFIED STRAIN AS YET UNNAMED) DNA POLYMERASE FRAGMENT

Released:
DOI: 10.2210/pdb1xwl/pdb
PDB entry 1xwl coloured by chain, front view.
PDB entry 1xwl coloured by chain, side view.
PDB entry 1xwl coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution.
Kiefer JR, Mao C, Hansen CJ, Basehore SL, Hogrefe HH, Braman JC, Beese LS
Structure 5 95-108 (1997)
PMID: 9016716

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52026 (Residues: 297-876; Coverage: 66%)
Gene names: pol, polA
Sequence domains: DNA polymerase family A
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 87.106Å b: 93.868Å c: 104.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.291
Expression system: Escherichia coli

Quick links

Citations

11 review citations

DNA replication fidelity.
Kunkel et al. (2000)
10 more

7 mentions without citation

Bst polymerase - a humble relative of Taq polymerase.
Oscorbin et al. (2023)
6 more