› 1xwv

X-ray diffraction
1.83Å resolution

Structure of the house dust mite allergen Der f 2: Implications for function and molecular basis of IgE cross-reactivity

Released:
DOI: 10.2210/pdb1xwv/pdb
PDB entry 1xwv coloured by chain, front view.
PDB entry 1xwv coloured by chain, side view.
PDB entry 1xwv coloured by chain, top view.
Source organism: Dermatophagoides farinae
Primary publication:
Structure of the house dust mite allergen Der f 2: implications for function and molecular basis of IgE cross-reactivity.
Johannessen BR, Skov LK, Kastrup JS, Kristensen O, Bolwig C, Larsen JN, Spangfort M, Lund K, Gajhede M
FEBS Lett 579 1208-12 (2005)
PMID: 15710415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mite group 2 allergen Der f 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.05 KDa
Source organism: Dermatophagoides farinae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q00855 (Residues: 18-146; Coverage: 100%)
Gene name: DERF2
Sequence domains: ML domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand PE3 1 x PE3
Ligand XPE 1 x XPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 25.712Å b: 47.638Å c: 48.275Å
α: 85.98° β: 75.9° γ: 82.76°
R-values:
R R work R free
0.221 0.221 0.257
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Hierarchy and molecular properties of house dust mite allergens.
Thomas WR. (2015)
4 more

10 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
9 more