› 1xxs

X-ray diffraction
1.8Å resolution

Structural insights for fatty acid binding in a Lys49 phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid

Released:
DOI: 10.2210/pdb1xxs/pdb
PDB entry 1xxs coloured by chain, front view.
PDB entry 1xxs coloured by chain, side view.
PDB entry 1xxs coloured by chain, top view.
Source organism: Bothrops moojeni
Primary publication:
Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid.
Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK
Biochimie 87 161-7 (2005)
PMID: 15760708

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 homolog myotoxin II Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.84 KDa
Source organism: Bothrops moojeni
UniProt:
  • Canonical: Q9I834 (Residues: 1-122; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand STE 6 x STE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P212121
Unit cell:
a: 61.18Å b: 88.71Å c: 51.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.166 0.247

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Citations

1 review citation

Catalytically Active Snake Venom PLA2 Enzymes: An Overview of Its Elusive Mechanisms of Reaction.
Castro-Amorim et al. (2023)
19 other articles

11 mentions without citation

phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine et al. (2010)
10 more