› 1y0q

X-ray diffraction
3.6Å resolution

Crystal structure of an active group I ribozyme-product complex

Released:
DOI: 10.2210/pdb1y0q/pdb
PDB entry 1y0q coloured by chain, front view.
PDB entry 1y0q coloured by chain, side view.
PDB entry 1y0q coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a phage Twort group I ribozyme-product complex.
Golden BL, Kim H, Chase E
Nat Struct Mol Biol 12 82-9 (2005)
PMID: 15580277

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195838 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
Group I ribozyme Chain: A
Molecule details ›
Chain: A
Length: 229 nucleotides
Theoretical weight: 73.93 KDa
Source organism: Staphylococcus virus Twort
Expression system: Escherichia coli
Sequence domains: Group I catalytic intron
5'-R(*GP*CP*UP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.22 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SPK 1 x SPK
Ligand MG 4 x MG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: I212121
Unit cell:
a: 94.57Å b: 140.97Å c: 210.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.277 0.31
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

36 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
35 more

34 mentions without citation

RNA structure: the long and the short of it.
Holbrook SR. (2005)
33 more