› 1y0y

X-ray diffraction
1.6Å resolution

Crystal structure of tetrahedral aminopeptidase from P. horikoshii in complex with amastatin

Released:
DOI: 10.2210/pdb1y0y/pdb
PDB entry 1y0y coloured by chain, front view.
PDB entry 1y0y coloured by chain, side view.
PDB entry 1y0y coloured by chain, top view.
Source organism: Streptomyces sp. ME98-M3
Primary publication:
Crystal structure of TET protease reveals complementary protein degradation pathways in prokaryotes.
Borissenko L, Groll M
J Mol Biol 346 1207-19 (2005)
PMID: 15713475

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129830 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetrahedral aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.07 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59196 (Residues: 1-353; Coverage: 100%)
Gene names: PH1527, frvX
Sequence domains: M42 glutamyl aminopeptidase
Structure domains:
AMASTATIN Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 475 Da
Source organism: Streptomyces sp. ME98-M3
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P23
Unit cell:
a: 111.56Å b: 111.56Å c: 111.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Peptidase inhibitors in the MEROPS database.
Rawlings ND. (2010)
30 other articles

4 mentions without citation

Target highlights in CASP9: Experimental target structures for the critical assessment of techniques for protein structure prediction.
Kryshtafovych et al. (2011)
3 more