› 1y10

X-ray diffraction
2.3Å resolution

Mycobacterial adenylyl cyclase Rv1264, holoenzyme, inhibited state

Released:
DOI: 10.2210/pdb1y10/pdb
PDB entry 1y10 coloured by chain, front view.
PDB entry 1y10 coloured by chain, side view.
PDB entry 1y10 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure of a pH-sensing mycobacterial adenylyl cyclase holoenzyme.
Tews I, Findeisen F, Sinning I, Schultz A, Schultz JE, Linder JU
Science 308 1020-3 (2005)
PMID: 15890882

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-161818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pH-sensitive adenylate cyclase Rv1264 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 407 amino acids
Theoretical weight: 43.5 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMU9 (Residues: 1-397; Coverage: 100%)
Gene names: MTCY50.18c, Rv1264
Sequence domains:
Structure domains: Nucleotide cyclase

Ligands and Environments

2 bound ligands:
Ligand CA 8 x CA
Ligand 1PE 3 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 61.936Å b: 146.83Å c: 84.849Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.193 0.19 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

Mycobacterium tuberculosis: success through dormancy.
Gengenbacher et al. (2012)
21 more

6 mentions without citation

Conditionally and transiently disordered proteins: awakening cryptic disorder to regulate protein function.
Jakob et al. (2014)
5 more