› 1y12

X-ray diffraction
1.95Å resolution

Structure of a hemolysin-coregulated protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb1y12/pdb
PDB entry 1y12 coloured by chain, front view.
PDB entry 1y12 coloured by chain, side view.
PDB entry 1y12 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 312 1526-30 (2006)
PMID: 16763151

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein hcp1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 165 amino acids
Theoretical weight: 17.99 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I747 (Residues: 1-162; Coverage: 100%)
Gene names: PA0085, hcp1
Sequence domains: Type VI secretion system effector, Hcp
Structure domains: Hcp1-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6
Unit cell:
a: 146.729Å b: 146.729Å c: 42.222Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

113 review citations

Type VII secretion--mycobacteria show the way.
Abdallah et al. (2007)
112 more

29 mentions without citation

A common evolutionary origin for tailed-bacteriophage functional modules and bacterial machineries.
Veesler et al. (2011)
28 more