› 1y23

X-ray diffraction
2.3Å resolution

Crystal structure of a member of HIT family of proteins from bacillus subtilis

Released:
DOI: 10.2210/pdb1y23/pdb
PDB entry 1y23 coloured by chain, front view.
PDB entry 1y23 coloured by chain, side view.
PDB entry 1y23 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Rajashankar KR, Lima CD, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein hit Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 145 amino acids
Theoretical weight: 16.32 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O07513 (Residues: 1-145; Coverage: 100%)
Gene names: BSU10030, hit, yhaE
Sequence domains: HIT domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 86.444Å b: 86.444Å c: 180.046Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.249
Expression system: Escherichia coli

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