› 1y3i

X-ray diffraction
2.6Å resolution

Crystal Structure of Mycobacterium tuberculosis NAD kinase-NAD complex

Released:
DOI: 10.2210/pdb1y3i/pdb
PDB entry 1y3i coloured by chain, front view.
PDB entry 1y3i coloured by chain, side view.
PDB entry 1y3i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex.
Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K
Biochem Biophys Res Commun 327 500-8 (2005)
PMID: 15629142

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.94 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHV7 (Residues: 1-307; Coverage: 100%)
Gene names: MTCI125.17, Rv1695, ppnK
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand GOL 4 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P62
Unit cell:
a: 110.45Å b: 110.45Å c: 108.932Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 not available 0.215
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
Pollak et al. (2007)
5 more

1 mention without citation

Conferring the ability to utilize inorganic polyphosphate on ATP-specific NAD kinase.
Nakamichi et al. (2013)