› 1y3n

X-ray diffraction
1.6Å resolution

Structure of AlgQ1, alginate-binding protein, complexed with an alginate disaccharide

Released:
DOI: 10.2210/pdb1y3n/pdb
PDB entry 1y3n coloured by chain, front view.
PDB entry 1y3n coloured by chain, side view.
PDB entry 1y3n coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Primary publication:
Direct evidence for Sphingomonas sp. A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: molecular insights into alginate transport in the periplasm.
Momma K, Mishima Y, Hashimoto W, Mikami B, Murata K
Biochemistry 44 5053-64 (2005)
PMID: 15794643

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Extracellular solute-binding protein Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 56.93 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT6 (Residues: 25-514; Coverage: 97%)
Gene name: algQ1
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: MAV, BEM
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 58.434Å b: 67.788Å c: 62.811Å
α: 90° β: 95.31° γ: 90°
R-values:
R R work R free
0.197 0.197 0.224
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Survey of the year 2005 commercial optical biosensor literature.
Rich et al. (2006)
5 more

5 mentions without citation

Conformational transitions upon ligand binding: holo-structure prediction from apo conformations.
Seeliger et al. (2010)
4 more