› 1y42

X-ray diffraction
1.95Å resolution

Crystal structure of a C-terminally truncated CYT-18 protein

Released:
DOI: 10.2210/pdb1y42/pdb
PDB entry 1y42 coloured by chain, front view.
PDB entry 1y42 coloured by chain, side view.
PDB entry 1y42 coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface.
Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM
Mol Cell 17 417-28 (2005)
PMID: 15694342

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase, mitochondrial Chain: X
Molecule details ›
Chain: X
Length: 392 amino acids
Theoretical weight: 44.92 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli
UniProt:
  • Canonical: P12063 (Residues: 33-423; Coverage: 58%)
Gene names: B18P24.070, NCU03030, cyt-18
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 1 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 104.88Å b: 73.21Å c: 56.79Å
α: 90° β: 111.35° γ: 90°
R-values:
R R work R free
0.179 0.174 0.235
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Atomic level architecture of group I introns revealed.
Vicens et al. (2006)
7 more

4 mentions without citation

DARS-RNP and QUASI-RNP: new statistical potentials for protein-RNA docking.
Tuszynska et al. (2011)
3 more