› 1y4c

X-ray diffraction
1.9Å resolution

Designed Helical Protein fusion MBP

Released:
DOI: 10.2210/pdb1y4c/pdb
PDB entry 1y4c coloured by chain, front view.
PDB entry 1y4c coloured by chain, side view.
PDB entry 1y4c coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
De novo design of an IL-4 antagonist and its structure at 1.9 A.
Laporte SL, Forsyth CM, Cunningham BC, Miercke LJ, Akhavan D, Stroud RM
Proc Natl Acad Sci U S A 102 1889-94 (2005)
PMID: 15684085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 494 amino acids
Theoretical weight: 54.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 69.557Å b: 74.69Å c: 103.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.225
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Insights into cytokine-receptor interactions from cytokine engineering.
Spangler et al. (2015)
4 more

6 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
5 more