› 1y6j

X-ray diffraction
3.01Å resolution

L-Lactate Dehydrogenase from Clostridium Thermocellum Cth-1135

Released:
DOI: 10.2210/pdb1y6j/pdb
PDB entry 1y6j coloured by chain, front view.
PDB entry 1y6j coloured by chain, side view.
PDB entry 1y6j coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Entry authors: Chen L, Yang H, Kataeva I, Chen LR, Tempel W, Lee D, Habel J, Zhou W, Lin D, Ljungdahl L, Liu Z-J, Rose J, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-107026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 34.87 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DCA4 (Residues: 1-318; Coverage: 100%)
Gene names: Cthe_0345, ldh
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P41212
Unit cell:
a: 73.36Å b: 73.36Å c: 191.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.229 0.271
Expression system: Escherichia coli

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