› 1y7b

X-ray diffraction
1.6Å resolution

BETA-D-XYLOSIDASE, A FAMILY 43 GLYCOSIDE HYDROLASE

Released:
DOI: 10.2210/pdb1y7b/pdb
PDB entry 1y7b coloured by chain, front view.
PDB entry 1y7b coloured by chain, side view.
PDB entry 1y7b coloured by chain, top view.
Source organism: Clostridium acetobutylicum ATCC 824
Entry authors: Teplyakov A, Fedorov E, Gilliland GL, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188893 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylosidase C-terminal Concanavalin A-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 542 amino acids
Theoretical weight: 62.42 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97DM1 (Residues: 2-532; Coverage: 100%)
Gene names: CA_C3452, xynD
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand SO4 6 x SO4
Ligand EPE 4 x EPE
Ligand PGE 1 x PGE
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 94.48Å b: 136.15Å c: 183.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.206
Expression system: Escherichia coli BL21

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