› 1y82

X-ray diffraction
2.3Å resolution

Conserved hypothetical protein Pfu-367848-001 from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1y82/pdb
PDB entry 1y82 coloured by chain, front view.
PDB entry 1y82 coloured by chain, side view.
PDB entry 1y82 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Entry authors: Horanyi P, Tempel W, Habel J, Chen L, Lee D, Nguyen D, Chang S-H, Florence Q, Zhou W, Lin D, Zhang H, Praissman J, Jenney Jr FE, Adams MWW, Liu Z-J, Rose JP, Wang BC, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VapC ribonuclease PF0355 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 18.46 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3V0 (Residues: 2-149; Coverage: 99%)
Gene name: PF0355
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

1 bound ligand:
Ligand UNX 42 x UNX
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 57.601Å b: 103.667Å c: 104.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.278
Expression system: Escherichia coli

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