› 1y8b

Solution NMR

Solution NMR-Derived Global Fold of Malate Synthase G from E.coli

Released:
DOI: 10.2210/pdb1y8b/pdb
PDB entry 1y8b coloured by chain, ensemble of 10 models, front view.
PDB entry 1y8b coloured by chain, ensemble of 10 models, side view.
PDB entry 1y8b coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution NMR-derived global fold of a monomeric 82-kDa enzyme.
Tugarinov V, Choy WY, Orekhov VY, Kay LE
Proc Natl Acad Sci U S A 102 622-7 (2005)
PMID: 15637152

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase G Chain: A
Molecule details ›
Chain: A
Length: 731 amino acids
Theoretical weight: 81.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 1-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing torsion angle dynamics
Expression system: Escherichia coli

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Citations

31 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
30 more

4 mentions without citation

Solution NMR of large molecules and assemblies.
Foster et al. (2007)
3 more