› 1yak

X-ray diffraction
2.5Å resolution

Complex of Bacillus subtilis TenA with 4-amino-2-methyl-5-hydroxymethylpyrimidine

Released:
DOI: 10.2210/pdb1yak/pdb
PDB entry 1yak coloured by chain, front view.
PDB entry 1yak coloured by chain, side view.
PDB entry 1yak coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.
Toms AV, Haas AL, Park JH, Begley TP, Ealick SE
Biochemistry 44 2319-29 (2005)
PMID: 15709744

Function and Biology Details

Reaction catalysed: 
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminopyrimidine aminohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 30.57 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25052 (Residues: 1-236; Coverage: 100%)
Gene names: BSU11650, tenA
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

1 bound ligand:
Ligand HMH 4 x HMH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2221
Unit cell:
a: 82.958Å b: 82.928Å c: 296.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.217 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The structural and biochemical foundations of thiamin biosynthesis.
Jurgenson et al. (2009)
3 more

5 mentions without citation

An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin.
Ng et al. (2019)
4 more