› 1ybe

X-ray diffraction
2.5Å resolution

Crystal Structure of a Nicotinate phosphoribosyltransferase

Released:
DOI: 10.2210/pdb1ybe/pdb
PDB entry 1ybe coloured by chain, front view.
PDB entry 1ybe coloured by chain, side view.
PDB entry 1ybe coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Entry authors: Seetharaman J, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 51.64 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8UIS9 (Residues: 1-434; Coverage: 100%)
Gene names: AGR_C_367, Atu0213, pncB
Sequence domains:
Structure domains: nicotinate phosphoribosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 179.768Å b: 69.428Å c: 87.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.272
Expression system: Escherichia coli

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