› 1yfp

X-ray diffraction
2.5Å resolution

STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP

Released:
DOI: 10.2210/pdb1yfp/pdb
PDB entry 1yfp coloured by chain, front view.
PDB entry 1yfp coloured by chain, side view.
PDB entry 1yfp coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein.
Wachter RM, Elsliger MA, Kallio K, Hanson GT, Remington SJ
Structure 6 1267-77 (1998)
PMID: 9782051

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.71 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 3-229; Coverage: 95%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 77.308Å b: 117.655Å c: 62.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
34 more

22 mentions without citation

Membrane-Mediated Oligomerization of G Protein Coupled Receptors and Its Implications for GPCR Function.
Gahbauer et al. (2016)
21 more