› 1yfu

X-ray diffraction
1.9Å resolution

Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from Ralstonia metallidurans

Released:
DOI: 10.2210/pdb1yfu/pdb
PDB entry 1yfu coloured by chain, front view.
PDB entry 1yfu coloured by chain, side view.
PDB entry 1yfu coloured by chain, top view.
Source organism: Cupriavidus metallidurans
Primary publication:
Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis.
Zhang Y, Colabroy KL, Begley TP, Ealick SE
Biochemistry 44 7632-43 (2005)
PMID: 15909978

Function and Biology Details

Reaction catalysed: 
3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyanthranilate 3,4-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.06 KDa
Source organism: Cupriavidus metallidurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1LCS4 (Residues: 1-174; Coverage: 100%)
Gene names: Rmet_5193, nbaC
Sequence domains: 3-hydroxyanthranilic acid dioxygenase
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand FE 2 x FE
Ligand CL 1 x CL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P6522
Unit cell:
a: 58.1Å b: 58.1Å c: 230.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.24
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
17 more

3 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
2 more