› 1yfz

X-ray diffraction
2.2Å resolution

Novel IMP Binding in Feedback Inhibition of Hypoxanthine-Guanine Phosphoribosyltransferase from Thermoanaerobacter tengcongensis

Released:
DOI: 10.2210/pdb1yfz/pdb
PDB entry 1yfz coloured by chain, front view.
PDB entry 1yfz coloured by chain, side view.
PDB entry 1yfz coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
Alternative IMP binding in feedback inhibition of hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis.
Chen Q, Liang Y, Su X, Gu X, Zheng X, Luo M
J Mol Biol 348 1199-210 (2005)
PMID: 15854655

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 22.76 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R7L0 (Residues: 1-181; Coverage: 100%)
Gene names: Hpt, TTE2394
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand MG 4 x MG
Ligand IMP 2 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2221
Unit cell:
a: 65.77Å b: 137.73Å c: 95.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.249
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex.
Endrizzi et al. (2005)
5 more

2 mentions without citation

Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase.
Monzani et al. (2007)
1 more