› 1yg2

X-ray diffraction
2.2Å resolution

Structure of the Vibrio cholerae virulence activator AphA

Released:
DOI: 10.2210/pdb1yg2/pdb
PDB entry 1yg2 coloured by chain, front view.
PDB entry 1yg2 coloured by chain, side view.
PDB entry 1yg2 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily.
De Silva RS, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ
J Biol Chem 280 13779-83 (2005)
PMID: 15647287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PadR family transcriptional regulator Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.45 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X399 (Residues: 1-179; Coverage: 100%)
Gene names: D6U24_09765, ERS013165_01671, ERS013200_00061, ERS013201_00878, EYB64_07510, F0M16_05160, FLM02_07620, FXE67_02205, aphA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 77.5Å b: 94.65Å c: 59.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.28
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Regulation of virulence by members of the MarR/SlyA family.
Ellison et al. (2006)
7 more

5 mentions without citation

Structure of the transcriptional regulator LmrR and its mechanism of multidrug recognition.
Madoori et al. (2009)
4 more