› 1yg9

X-ray diffraction
1.3Å resolution

The structure of mutant (N93Q) of bla g 2

Released:
DOI: 10.2210/pdb1yg9/pdb
PDB entry 1yg9 coloured by chain, front view.
PDB entry 1yg9 coloured by chain, side view.
PDB entry 1yg9 coloured by chain, top view.
Source organism: Blattella germanica
Primary publication:
Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition.
Gustchina A, Li M, Wünschmann S, Chapman MD, Pomés A, Wlodawer A
J Mol Biol 348 433-44 (2005)
PMID: 15811379

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Aspartic protease Bla g 2 Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.18 KDa
Source organism: Blattella germanica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P54958 (Residues: 25-350; Coverage: 98%)
Sequence domains: Eukaryotic aspartyl protease
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 141.6Å b: 38.6Å c: 71.5Å
α: 90° β: 100.9° γ: 90°
R-values:
R R work R free
0.181 0.18 0.212
Expression system: Komagataella pastoris

Quick links

Citations

13 review citations

Nomenclature and structural biology of allergens.
Chapman et al. (2007)
12 more

16 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
15 more