› 1yio

X-ray diffraction
2.2Å resolution

Crystallographic structure of response regulator StyR from Pseudomonas fluorescens

Released:
DOI: 10.2210/pdb1yio/pdb
PDB entry 1yio coloured by chain, front view.
PDB entry 1yio coloured by chain, side view.
PDB entry 1yio coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
An active-like structure in the unphosphorylated StyR response regulator suggests a phosphorylation- dependent allosteric activation mechanism.
Milani M, Leoni L, Rampioni G, Zennaro E, Ascenzi P, Bolognesi M
Structure 13 1289-97 (2005)
PMID: 16154086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory protein Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.4 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: O30989 (Residues: 1-208; Coverage: 100%)
Gene name: styR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HG 3 x HG
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 41.196Å b: 35.679Å c: 73.877Å
α: 90° β: 99.15° γ: 90°
R-values:
R R work R free
0.205 0.201 0.275
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial response regulators: versatile regulatory strategies from common domains.
Gao et al. (2007)
3 more

4 mentions without citation

Receiver domain structure and function in response regulator proteins.
Bourret RB. (2010)
3 more