› 1yiw

X-ray diffraction
1.39Å resolution

X-ray Crystal Structure of a Chemically Synthesized Ubiquitin

Released:
DOI: 10.2210/pdb1yiw/pdb
PDB entry 1yiw coloured by chain, front view.
PDB entry 1yiw coloured by chain, side view.
PDB entry 1yiw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Bang D, Makhatadze GI, Tereshko V, Kossiakoff AA, Kent SB
Angew Chem Int Ed Engl 44 3852-6 (2005)
PMID: 15834850

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-192942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9PST8 (Residues: 1-71; Coverage: 100%)
Gene name: UbI
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand CD 17 x CD
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 43.776Å b: 50.476Å c: 92.649Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.207
Expression system: Not provided

Quick links

Citations

12 review citations

Chemoselective ligation and modification strategies for peptides and proteins.
Hackenberger et al. (2008)
11 more

11 mentions without citation

Structural perspective on mutations affecting the function of multisubunit RNA polymerases.
Trinh et al. (2006)
10 more