› 1yjd

X-ray diffraction
2.7Å resolution

Crystal structure of human CD28 in complex with the Fab fragment of a mitogenic antibody (5.11A1)

Released:
DOI: 10.2210/pdb1yjd/pdb
Model geometry
Fit model/data
PDB entry 1yjd coloured by chain, front view.
PDB entry 1yjd coloured by chain, side view.
PDB entry 1yjd coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a soluble CD28-Fab complex.
Evans EJ, Esnouf RM, Manso-Sancho R, Gilbert RJ, James JR, Yu C, Fennelly JA, Vowles C, Hanke T, Walse B, Hünig T, Sørensen P, Stuart DI, Davis SJ
Nat Immunol 6 271-9 (2005)
PMID: 15696168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210491 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab fragment of 5.11A1 antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.39 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment of 5.11A1 antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 24.11 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
T-cell-specific surface glycoprotein CD28 Chain: C

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2
Unit cell:
a: 191.219Å b: 47.417Å c: 71.842Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.239 0.239 0.282
Expression system: Cricetulus griseus

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Citations

31 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
30 more

42 mentions without citation

Stapled Peptides Inhibitors: A New Window for Target Drug Discovery.
Ali et al. (2019)
41 more