› 1ykj

X-ray diffraction
2Å resolution

A45G p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound

Released:
DOI: 10.2210/pdb1ykj/pdb
PDB entry 1ykj coloured by chain, front view.
PDB entry 1ykj coloured by chain, side view.
PDB entry 1ykj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Removal of a methyl group causes global changes in p-hydroxybenzoate hydroxylase.
Cole LJ, Gatti DL, Entsch B, Ballou DP
Biochemistry 44 8047-58 (2005)
PMID: 15924424

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 44.37 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P20586 (Residues: 1-394; Coverage: 100%)
Gene names: PA0247, pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand SO4 12 x SO4
Ligand PHB 2 x PHB
Ligand PSL 2 x PSL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P21212
Unit cell:
a: 69.86Å b: 85.897Å c: 146.074Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.265
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Dynamics involved in catalysis by single-component and two-component flavin-dependent aromatic hydroxylases.
Ballou et al. (2005)
1 more

3 mentions without citation

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
2 more