› 1ylf

X-ray diffraction
2.5Å resolution

X-ray crystal structure of BC1842 protein from Bacillus cereus, a member of the Rrf2 family of putative transcription regulators.

Released:
DOI: 10.2210/pdb1ylf/pdb
PDB entry 1ylf coloured by chain, front view.
PDB entry 1ylf coloured by chain, side view.
PDB entry 1ylf coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Entry authors: Osipiuk J, Wu R, Moy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
homo hexamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RRF2 family protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81EX1 (Residues: 1-146; Coverage: 100%)
Gene name: BC_1842
Sequence domains: Iron-dependent Transcriptional regulator
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand CL 5 x CL
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 73.08Å b: 73.08Å c: 157.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.217 0.25
Expression system: Escherichia coli BL21(DE3)

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