› 1yo7

X-ray diffraction
2.8Å resolution

Re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle

Released:
DOI: 10.2210/pdb1yo7/pdb
PDB entry 1yo7 coloured by chain, front view.
PDB entry 1yo7 coloured by chain, side view.
PDB entry 1yo7 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Sagermann M, Emery SC, Sander C

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P03051 (Residues: 1-61, 32-57, 2-29; Coverage: 97%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Alpha-catenin/vinculin-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21
Unit cell:
a: 46.607Å b: 52.83Å c: 50.36Å
α: 90° β: 94.99° γ: 90°
R-values:
R R work R free
0.224 0.216 0.31
Expression system: Escherichia coli K-12

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