› 1yqv

X-ray diffraction
1.7Å resolution

The crystal structure of the antibody Fab HyHEL5 complex with lysozyme at 1.7A resolution

Released:
DOI: 10.2210/pdb1yqv/pdb
PDB entry 1yqv coloured by chain, front view.
PDB entry 1yqv coloured by chain, side view.
PDB entry 1yqv coloured by chain, top view.
Source organisms:
Primary publication:
Water molecules in the antibody-antigen interface of the structure of the Fab HyHEL-5-lysozyme complex at 1.7 A resolution: comparison with results from isothermal titration calorimetry.
Cohen GH, Silverton EW, Padlan EA, Dyda F, Wibbenmeyer JA, Willson RC, Davies DR
Acta Crystallogr D Biol Crystallogr 61 628-33 (2005)
PMID: 15858274

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208254 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HyHEL-5 Antibody Light Chain Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 23.23 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
HyHEL-5 Antibody Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 215 amino acids
Theoretical weight: 23.03 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Lysozyme C Chain: Y
Molecule details ›
Chain: Y
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 54.459Å b: 74.258Å c: 78.256Å
α: 90° β: 101.65° γ: 90°
R-values:
R R work R free
0.195 0.195 0.234

Quick links

Citations

4 review citations

The structural basis of antibody-antigen recognition.
Sela-Culang et al. (2013)
3 more

31 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
30 more