› 1yrx

X-ray diffraction
2.3Å resolution

Structure of a novel photoreceptor: the BLUF domain of AppA from Rhodobacter sphaeroides

Released:
DOI: 10.2210/pdb1yrx/pdb
PDB entry 1yrx coloured by chain, front view.
PDB entry 1yrx coloured by chain, side view.
PDB entry 1yrx coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
Structure of a novel photoreceptor, the BLUF domain of AppA from Rhodobacter sphaeroides.
Anderson S, Dragnea V, Masuda S, Ybe J, Moffat K, Bauer C
Biochemistry 44 7998-8005 (2005)
PMID: 15924418

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-176042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 121 amino acids
Theoretical weight: 13.54 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53119 (Residues: 17-133; Coverage: 26%)
Gene name: appA
Sequence domains: Sensors of blue-light using FAD
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand FMN 3 x FMN
Ligand D9G 6 x D9G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P41212
Unit cell:
a: 98.671Å b: 98.671Å c: 127.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.273
Expression system: Escherichia coli

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Citations

18 review citations

Structure and function of plant photoreceptors.
Möglich et al. (2010)
17 more

28 mentions without citation

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
27 more