› 1ys3

X-ray diffraction
1.9Å resolution

Crystal Structure of the ATP binding domain of PrrB from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb1ys3/pdb
PDB entry 1ys3 coloured by chain, front view.
PDB entry 1ys3 coloured by chain, side view.
PDB entry 1ys3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Nowak E, Panjikar S, Tucker P, Mycobacterium Tuberculosis Structural Proteomics Project (XMTB)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor-type histidine kinase PrrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 150 amino acids
Theoretical weight: 15.25 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGK7 (Residues: 297-446; Coverage: 34%)
Gene names: MTCY31.30c, Rv0902c, prrB
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand TRS 3 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1, EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P65
Unit cell:
a: 61.476Å b: 61.476Å c: 211.333Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.235
Expression system: Escherichia coli

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